Mouse anti-CETP(N) mAb
Mouse anti-CETP(N) monoclonal antibody (Catalog # TVBP003) for cholesterol ester transfer protein assay.
Specificity: Mouse anti-CETP(N) monoclonal antibody against N-terminal amino acid residues 1 to 12 of human cholesterol ester transfer protein.
Antigen used for immunizations: Prokaryotic recombinant protein containing 7 copies of the N-terminal amino acid residues 1 to 12 of the cholesterol ester transfer protein in linear array was used as immunogen for immunization.
Storage Buffer: The mouse anti-CETP(N) monoclonal antibody (Catalog # TVBP003) for cholesterol ester transfer protein is kept in PBS.
Western blotting Recommended:
Working dilution for Western blotting 1:1000. Primary antibody incubate at 25℃ for 60min.
Figure 1. Western blotting of human serum cholesterol ester transfer protein with mouse anti-CETP(N) monoclonal antibody (Catalog # TVBP003).
Storage and stability: Store unopened antibody at -20℃ or lower. Under these conditions, there is no significant loss in product performance up to one year from the date of purchase. The antibody is stable for at least two months when stored at 4℃. For long term storage, it is recommended that aliquots of the antibody are frozen at -20℃ (frost-free freezers are not recommended). Repeated freezing and thawing must be avoided. Prepare working dilutions on the day of use.
Legal consideration: FOR RESEARCH USE ONLY.
Application: Cholesterol ester transfer protein (CETP) is a serum enzyme involved in a variety of activities such as catalysis of the non-directional transfer of neutral lipids, mainly cholesteryl ester (CE) and triglyceride (TG), between lipoproteins in plasma. As a result, the levels of CETP may decide the profile of lipoproteins such as HDL, LDL VLDL and chylomicrons in serum. Thus, the regulation of the activities of CETP may be a promising strategy for increasing HDL-C levels and CETP may be used to a possible target to treat atherosclerotic cardiovascular disease and fatty liver.
References:
Liaw Yi-Wei, Lin Chi-Yu, Lai Yu-Sheng, Yang Tzu-Chung, Wang Chau-Jong, Whang-Peng J., Liu Leroy F, Lin Chia-Po, Nieh Shin, Lu Shao-Chun, Hwang J. A vaccine targeted at CETP alleviates high fat and high cholesterol diet-induced atherosclerosis and non-alcoholic steatohepatitis in rabbit. PLoS ONE; 2014, 9(12): e111529. doi:10.1371/journal.pone.0111529
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A biologics targeted at CETP alleviates high fat and high cholesterol diet-induced atherosclerosis and non-alcoholic steatohepatitis (NASH) in rabbit. Low HDL-C levels are associated with atherosclerosis and non-alcoholic steatohepatitis, and increased levels may reduce the risk of these diseases. Inhibition of cholesteryl ester transfer protein (CETP) activity is considered a promising strategy for increasing HDL-C levels. Since CETP is a self-antigen with low immunogenicity, Taivital Biopharmaceutical Co. LTD. have successfully developed a novel anti-CETP biologics (Fc-CETP6) to overcome the low immunogenicity of CETP and for long-term inhibition of CETP activity. The anti-CETP biologics consists of a rabbit IgG Fc domain for antigen delivery to antigen-presenting cells fused to a linear array of 6 repeats of a CETP epitope to efficiently activate B cells. Rabbits were fed a high fat/cholesterol (HFC) diet to induce atherosclerosis and NASH, and immunized with Fc-CETP6 anti-CETP biologics. The Fc-CETP6 anti-CETP biologics successfully elicited anti-CETP antibodies and lowered plasma CETP activity. The levels of plasma HDL-C and ApoA-I were higher, and plasma ox-LDL lower, in the Fc-CETP6-immunized rabbits as compared to the unimmunized HFC diet-fed rabbits. Pathological analyses revealed less lipid accumulation and inflammation in the aorta and liver of the Fc-CETP6-immunized rabbits. These results show that the Fc-CETP6 anti-CETP biologics efficiently elicited antibodies against CETP and reduced susceptibility to both atherosclerosis and steatohepatitis induced by the HFC diet. Our findings suggest that the Fc-CETP6 anti-CETP biologics may improve atherosclerosis and NASH and has high potential for clinical use. Reference:Liaw Yi-Wei, Lin Chi-Yu, Lai Yu-Sheng, Yang Tzu-Chung, Wang Chau-Jong, Whang-Peng J., Liu Leroy F, Lin Chia-Po, Nieh Shin, Lu Shao-Chun, Hwang J. A vaccine targeted at CETP alleviates high fat and high cholesterol diet-induced atherosclerosis and non-alcoholic steatohepatitis in rabbit. PLoS ONE; 2014, 9(12): e111529. doi:10.1371/journal.pone.0111529
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Mouse anti-CETP(C) monoclonal antibody (Catalog #TVBP004) for cholesterol ester transfer protein assay Specificity: Mouse anti-CETP(C) monoclonal antibody against C-terminal amino acid residues 463 to 475 of human cholesterol ester transfer protein. Antigen used for immunizations: Prokaryotic recombinant protein containing 7 copies of the C-terminal amino acid residues 463-475 of the cholesterol ester transfer protein in linear array. Storage Buffer: The mouse anti-CETP(C) monoclonal antibody (Catalog #TVBP004) against human cholesterol ester transfer protein is kept in PBS. Western blotting Recommended: Working dilution for Western blotting 1:1000. Primary antibody incubate at 25℃ for 60min. Figure 1. Western blotting of human serum cholesterol ester transfer protein Storage and stability: Store unopened antibody at -20℃ or lower. Under these conditions, there is no significant loss in product performance up to one year from the date of purchase. The antibody is stable for at least two months when stored at 4℃. For long term storage, it is recommended that aliquots of the antibody are frozen at -20℃ (frost-free freezers are not recommended). Repeated freezing and thawing must be avoided. Prepare working dilutions on the day of use. Legal consideration: FOR RESEARCH USE ONLY. Application: Cholesterol ester transfer protein (CETP) is a serum enzyme involved in a variety of activities such as catalysis of the non-directional transfer of neutral lipids, mainly cholesteryl ester (CE) and triglyceride (TG), between lipoproteins in plasma. As a result, the levels of CETP may decide the profile of lipoproteins such as HDL, LDL VLDL and chylomicrons in serum. Thus, controlling the levels of CETP may be a good approach to affect the rise of HDL-C and may be a possible target to treat with atherosclerotic cardiovascular disease and fatty liver. References: Liaw Yi-Wei, Lin Chi-Yu, Lai Yu-Sheng, Yang Tzu-Chung, Wang Chau-Jong, Whang-Peng J., Liu Leroy F, Lin Chia-Po, Nieh Shin, Lu Shao-Chun, Hwang J. A vaccine targeted at CETP alleviates high fat and high cholesterol diet-induced atherosclerosis and non-alcoholic steatohepatitis in rabbit. PLoS ONE; 2014, 9(12): e111529. doi:10.1371/journal.pone.0111529
