Containing orange G as tracking dyesCat. No. DL0507Size: 50μg / 500μlStore at -20°CDescriptionA unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 17 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 50-1,500 base pairs. The 200 and 500 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg a load) for approximating the mass of DNA in comparably intense samples of similar size.Source: PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 1mM EDTA. Range: 50-1,500 bpNumber of bands: 17Concentration: 100 μg/mlRecommended Load: 5 μl / well

Cat. No. DL0508Size: 50 μg (500 μl)Store at -20°CDescriptionThe 0.25-25Kb DNA Ladder is composed of 14 individual DNA fragments: 25K, 10K, 8K, 6K, 5K, 4K, 3K, 2.5K, 2K, 1500, 1000, 750, 500, 250 base pairs. This product contains two enhanced bands (3K and 1K bp) for easy reference. The ladder is ready-to-use, which is premixed with loading buffer dye for direct loading on gel. Source:PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 1mM EDTA.Range: 250-25K bpNumber of bands: 14Concentration: 100 μg/mlRecommended Load:0.5 μg (5 μl)

Cat. No. DL0501Size: 50 μg (500 μl)Store at -20°CDescription A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 11 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 100-1,500 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg a load) for approximating the mass of DNA in comparably intense samples of similar size.Source: PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 1mM EDTA.Range: 100-1,500 bpNumber of bands: 11Concentration: 100 μg/mlRecommended Load: 0.5 μg (5 μl)

BM-Bead Genomic DNA Kit (Blood)            Cat. No. BM0100 Sample: up to 200 μl of whole blood For research use only Introduction The BM-Bead Genomic DNA Kit provides a fast, simple, and cost-effective method for isolation of genomic DNA from blood cells. Its unique buffer system will efficiently lyse cells and degrade protein, allowing for DNA to be easily bound by the surface of the magnetic beads. Phenol extraction and ethanol precipitation are not required, and high-quality genomic DNA is eluted in the BM Release Buffer. Genomic DNA purified with BM-Bead Genomic DNA Kit is suitable for a variety of routine applications. The entire procedure can be completed within 15-20 minutes (using most magnetic separators) or the kit can be easily adapted to satisfy most automated Nucleic Acid purification systems.

CM-Bead Genomic DNA Kit  (Buffy Coat) Cat. No. CM0100 ample: up to 300 μl of Buffy Coat For research use onlyDescription CM-Bead Genomic DNA kit (Buffy Coat) was designed specifically for genomic DNA isolation from Buffy Coat samples. Its special buffer system will efficiently lyse cell and degrade protein, allowing for DNA to be easily bound by the surface of the magnetic beads. The other non-specific binding particles are removed with a wash buffer, and the genomic DNA is released from the beads by addition of a low ionic strength buffer and heat. Genomic DNA can be purified manually within 15 minutes (using most magnetic separators) or the kit can be easily adapted to satisfy most automated Nucleic Acid purification systems.

TM-Bead Genomic DNA Kit (Tissue)  Cat. No.TM0100 Sample: up to 30 mg of tissue For research use only Introduction This magnetic bead genomic DNA purification kit was designed specifically for genomic DNA isolation from animal tissue samples. Its unique buffer system will efficiently lyse cells and degrade protein, allowing for DNA to be easily bound by the surface of the magnetic beads. RNA and other non-specific binding particles are removed with a wash buffer, and the genomic DNA is then released into the Release Buffer. Genomic DNA can be purified manually within 50 minutes (using most magnetic separators) or the kit can be easily adapted to satisfy most automated Nucleic Acid purification systems.

EM-Bead Genomic DNA Kit  (Bacteria) Cat. No.EM0100 Sample: up to 300 μl of bacteria sample For research use only Introduction EM-Bead Genomic DNA kit (Bacteria) was designed specifically for genomic DNA isolation from bacteria samples. Its special buffer system will efficiently lyse cell and degrade protein, allowing for DNA to be easily bound by the surface of the magnetic beads. The other non-specific binding particles are removed with a wash buffer, and the genomic DNA is released from the beads by addition of a low ionic strength buffer and heat. Genomic DNA can be purified manually within 15 minutes (using most magnetic separators) or the kit can be easily adapted to satisfy most automated Nucleic Acid purification systems.

Cat. No. VM0100 Sample: up to 300 μl of virus sample For research use only Introduction This magnetic bead genomic DNA purification kit was designedspecifically for simultaneous virus DNA/RNA purification from plasma, serum, body fluid or the supernatant of viral infected cell cultures. Its unique buffer system will efficiently lyse cells and degrade protein, allowing for Nucleic Acid to be easily bound by the surface of the magnetic beads. The other non-specific binding particles are removed with a wash buffer, and the genomic Nucleic Acid is then released into the Release Buffer. Genomic Nucleic Acid can be purified manually within 10-15 minutes (using most magnetic separators) or the kit can be easily adapted to satisfy most automated Nucleic Acid purification systems.

BM-Bead Genomic DNA Kit (Blood)            Cat. No. BM0100 Sample: up to 200 μl of whole blood For research use only Introduction The BM-Bead Genomic DNA Kit provides a fast, simple, and cost-effective method for isolation of genomic DNA from blood cells. Its unique buffer system will efficiently lyse cells and degrade protein, allowing for DNA to be easily bound by the surface of the magnetic beads. Phenol extraction and ethanol precipitation are not required, and high-quality genomic DNA is eluted in the BM Release Buffer. Genomic DNA purified with BM-Bead Genomic DNA Kit is suitable for a variety of routine applications. The entire procedure can be completed within 15-20 minutes (using most magnetic separators) or the kit can be easily adapted to satisfy most automated Nucleic Acid purification systems.

Cat. No. VL0100  Sample:  up to  1 ml of virus sample       For research use only Introduction The Total Nucleic Acid Isolation Kit (Virus)-L provides a fast, simple, and cost-effective method for isolation of viral DNA/RNA from cell-free samples such as serum, plasma, body fluids and the supernatant of viral infected cell cultures. Its unique buffer system will efficiently lyse cells and degrade protein, allowing for nucleic acid to be easily bound by the glass fiber matrix of the column . Contaminants such as salts, metabolites and soluble macromolecular cellular components are removed in the Wash step. Phenol extraction and ethanol precipitation are not required, and high-quality Nucleic Acid is eluted in RNase-free elution buffer. Viral DNA/RNA isolated with The Total Nucleic Acid Isolation Kit (Virus)-L is suitable for a variety of routine applications, including Real-time PCR/RT-PCR, Automated Fluorescent DNA Sequencing, PCR, and other enzymatic reactions. The entire procedure can be completed within 45-50 minutes.

Cat. No. VN0100 Sample:  up to  200 μl of virus sample       For research use only Introduction The Total Nucleic Acid Isolation Kit (Virus) provides a fast, simple, and cost-effective method for isolation of viral DNA/RNA from cell-free samples such as serum, plasma, body fluids and the supernatant of viral infected cell cultures. Its unique buffer system will efficiently lyse cells and degrade protein, allowing for nucleic acid to be easily bound by the glass fiber matrix of the column . Contaminants such as salts, metabolites and soluble macromolecular cellular components are removed in the Wash step. Phenol extraction and ethanol precipitation are not required, and high-quality Nucleic Acid is eluted in RNase-free elution buffer. Viral DNA/RNA isolated with The Total Nucleic Acid Isolation Kit (Virus) is suitable for a variety of routine applications, including Real-time PCR/RT-PCR, Automated Fluorescent DNA Sequencing, PCR, and other enzymatic reactions. The entire procedure can be completed within 15-20 minutes.

Cat. No. PQ0100 Size: 100 reactions (4 X 1.125ml)Store at -20°CDescription PCR All in One contains Mg++, dNTPs, and recombinant Taq DNA Polymerase at concentrations sufficient to allow amplification during PCR.PCR All in One may be stored at either -20°C or 4°C. Storage at 4°C avoids the necessity of thawing the mix before assembling the PCR. No detectable reduction of PCR performance or enzyme activity is observed after storage of PCR All in One for 12 months at 4°C. Repeated freeze-thaw cycles do not reduce performance. For research use only

Cat. No. HQ0100 Size: 100 reactionsStore at 4 or -20°CDescription HotStar All in One provides qualified reagents for the amplification of nucleic acid templates by polymerase chain reaction (PCR). The mixture contains anti-Taq DNA polymerase antibody, Mg++, dNTPs, and recombinant Taq DNA polymerase at concentrations sufficient to allow amplification during PCR. HotStar All in One is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 100 amplification reactions of 50 μl each are provided. Due to specific binding of the antibody, HotStar All in One is present in an inactive form and is reactivated after a denaturation step in PCR cycling at 94°C. HotStar All in One may be stored at either -20°C or 4°C. No detectable reduction of PCR performance or enzyme activity is observed after storage of HotStar All in One for 12 months at 4°C. Repeated freeze-thaw cycles do not reduce performance or activity.

Cat. No. HI0100 Size: 100 Reactions  Conc. 1 U/μl   Store at -20°CDescription HiFi is a new recombinant enzyme with genetic modification for its amino acid sequence, which results 70 times better fidelity than Taq DNA polymerase and an extremely fast elongation rate (as fast as 15 seconds per kb). HiFi has higher stability at high temperature. Users may program the initial denaturizing at even 100°C for 2 min, and this property makes HiFi perform very well for GC-rich PCR. NoteHiFi DNA Polymerase produces blunt end PCR products. Add conventional Taq polymerase at the final step for further TA or GC cloning application.

Cat No. TQ0500 Size500 units Conc.5 units/μlStore at -20°C  Description Taq DNA Polymerase is purified from E. coli. expressing a Thermus aquaticus DNA polymerase gene. This enzyme has a 5' → 3' DNA polymerase and a 5' → 3' exonuclease activity but lacks a 3' → 5' exonuclease activity. Taq DNA polymerase is heat-stable and will synthesize DNA at elevated temperatures from single- stranded templates in the presence of a primer.  Contents Taq DNA Polymerase 100 ul(5 units/ul) 10X PCR buffer 1.5ml

Cat. No. PL0500 Size: 500 μlStore at -20°C for 24 months          • 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15 well or 10                         well mini-gel  respectively.          • 2~3 μl per well for general Western transfering.         • Apply more for thicker (>1.5 mm) or larger gel. DescriptionThe 10-180kDa Prestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The 10-180kDa Prestained Protein Ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes(PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading. ContentsApproximately 0.2~0.4 mg/ml of each protein in buffer (20 mM Trisphosphate pH 7.5 at 25°C), 2% SDS, 1 mM 2-Mercaptoethanol, 3.6 M Urea, and 15% (v/v) glycerol). Quality Control 5 μl of 10-180kDa Prestained Protein Ladder resolves 10 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane.NoteThe apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in eachelectrophoresis condition

Cat. No. PL0501                                      Size: 500 μl Store at -20°C • 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15 well or 10 well  mini-gel    respectively.• 1.5~2.5 μl per well for general Western transferring.• Apply more for thicker (> 1.5 mm) or larger gel.Description The10-245kDa Prestained Protein Ladderis a three-color protein standard with 12 pre stained proteins covering a wide range molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The 10-245kDa Prestained Protein Ladderis designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Contents Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Trisphosphate, pH 7.5 at 25°C), 2 % SDS, 3.6 M Urea,1 mM 2-Mercaptoethanol,  and 15 % (v/v) Glycerol).Quality Control Under suggested conditions, 10-245kDa Prestained Protein Ladder resolves 12 major bands in 15% SDS-PAGE (Tris-glycine buffer) and after Western blotting to nitrocellulose membrane.Note The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in eachelectrophoresis condition

Cat. No. PL0502 Size: 500 μl Store at -20°C        • 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15 well or 10            well mini-gel respectively.        • 1.5~2.5 μl per well for general Western transferring.        • Apply more for thicker (> 1.5 mm) or larger gel.Description The 3.5-245kDa Prestained Protein Ladderis a three-color protein standard with 13 pre-stained proteins covering a wide range molecular weights from 3.5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).The 3.5-245 kDa Prestained Protein Ladderis designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of  Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.Contents Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Trisphosphate, pH 7.5 at 25°C), 2 % SDS, 10 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).Quality Control Under suggested conditions, 3.5-245kDa Prestained Protein Ladderresolves 13 major bands in 15% SDS-PAGE (Tris-glycine buffer) and after Western blotting to nitrocellulose membrane.

Total RNA Isolation Kit (Blood/Cell/Bacteria)                        For research use only                                                            Sample: up to 300 μl of whole blood, 107mammalian cells and 109 bacterial cells Yield: up to 30μg Introduction The Total RNA Isolation Kit provides a fast, simple, and cost-effective method for isolation of total RNA from whole blood, mammalian cells and bacterial cells. Detergents and chaotropic salt are used to lyse cells and inactivate RNase. The specialized high-salt buffering system allows RNA species bases to bind to the the glass fiber matrix of the spin column (1) while contaminants pass through the column. Impurities are efficiently washed away, and the pure RNA is eluted with REL Buffer without phenol extraction or alcohol precipitation. RNA purified with The Total RNA Isolation Kit is suitable for a variety of routine applications including RT-PCR, cDNA Synthesis, Northern Blotting, Differential display, Primer Extension and mRNA Selection .The entire procedure can be completed within 25-40 minutes.

Total RNA Isolation Kit (Tissue)                                                 For research use only                                                            Sample: up to 30 mg of tissue, up to 25 mg of paraffin-embedded tissue Yield  : up to 30μg Introduction The Total RNA Isolation Kit provides a fast, simple, and cost-effective method for isolation of total RNA from tissue sample. Detergents and chaotropic salt are used to lyse cells and inactivate RNase. The specialized high-salt buffering system allows RNA species bases to bind to the the glass fiber matrix of the spin column (1) while contaminants pass through the column. Impurities are efficiently washed away, and the pure RNA is eluted with REL Buffer without phenol extraction or alcohol precipitation. RNA purified with The Total RNA Isolation Kit is suitable for a variety of routine applications including RT-PCR, cDNA Synthesis, Northern Blotting, Differential display, Primer Extension and mRNA Selection. The entire procedure can be completed within 25-40 minutes.