Cat. No. PL0502 Size: 500 μl Store at -20°C• 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15 well or 10 well mini-gel respectively.• 1.5~2.5 μl per well for general Western transferring.• Apply more for thicker (> 1.5 mm) or larger gel.Description The 3.5-245kDa Prestained Protein Ladderis a three-color protein standard with 13 pre-stained proteins covering a wide range molecular weights from 3.5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).The 3.5-245 kDa Prestained Protein Ladderis designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.Contents Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Trisphosphate, pH 7.5 at 25°C), 2 % SDS, 10 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).Quality Control Under suggested conditions, 3.5-245kDa Prestained Protein Ladderresolves 13 major bands in 15% SDS-PAGE (Tris-glycine buffer) and after Western blotting to nitrocellulose membrane.
Total RNA Isolation Kit (Blood/Cell/Bacteria) For research use only Sample: up to 300 μl of whole blood, 107mammalian cells and 109 bacterial cells Yield: up to 30μg Introduction The Total RNA Isolation Kit provides a fast, simple, and cost-effective method for isolation of total RNA from whole blood, mammalian cells and bacterial cells. Detergents and chaotropic salt are used to lyse cells and inactivate RNase. The specialized high-salt buffering system allows RNA species bases to bind to the the glass fiber matrix of the spin column (1) while contaminants pass through the column. Impurities are efficiently washed away, and the pure RNA is eluted with REL Buffer without phenol extraction or alcohol precipitation. RNA purified with The Total RNA Isolation Kit is suitable for a variety of routine applications including RT-PCR, cDNA Synthesis, Northern Blotting, Differential display, Primer Extension and mRNA Selection .The entire procedure can be completed within 25-40 minutes.
Total RNA Isolation Kit (Tissue) For research use only Sample: up to 30 mg of tissue, up to 25 mg of paraffin-embedded tissue Yield : up to 30μg Introduction The Total RNA Isolation Kit provides a fast, simple, and cost-effective method for isolation of total RNA from tissue sample. Detergents and chaotropic salt are used to lyse cells and inactivate RNase. The specialized high-salt buffering system allows RNA species bases to bind to the the glass fiber matrix of the spin column (1) while contaminants pass through the column. Impurities are efficiently washed away, and the pure RNA is eluted with REL Buffer without phenol extraction or alcohol precipitation. RNA purified with The Total RNA Isolation Kit is suitable for a variety of routine applications including RT-PCR, cDNA Synthesis, Northern Blotting, Differential display, Primer Extension and mRNA Selection. The entire procedure can be completed within 25-40 minutes.
Total RNA Isolation Kit (Plant) For research use only Sample: 100 mg of fresh plant tissue or 25 mg of dry plant tissue Yield: up to 30μg IntroductionThe Total RNA Isolation Kit provides a fast, simple, and cost-effective method for isolation of total RNA from plant samples. Detergents and chaotropic salt are used to lyse cells and inactivate RNase. The specialized high-salt buffering system allows RNA species longer than 100 bases to bind to the the glass fiber matrix of the spin column . The Total RNA Isolation Kit is suitable for a variety of routine applications including RT-PCR, cDNA Synthesis, Northern Blotting, Differential display, Primer Extension and mRNA Selection . The entire procedure can be completed within 60 minutes.
DNA Isolation Dual Kit (Blood/Cultured Cell) For research use only Sample: up to 300 μl of whole blood, 200 μl of buffy coat, 107mammalian cells, 5×107 fungus cells and 109 bacterial cells Yield: up to 50μg Introduction The DNA Isolation Dual Kit (Blood/Cultured Cell) combines reagent system and spin column system in it. The kit was designed specifically for DNA isolation from whole blood, frozen blood, buffy coat, cultured animal/bacterial cells and fungus. This unique reagent system ensures DNA with high yield and good quality from samples and the spin column system was designed to purify or concentrate DNA products which have been previously isolated using Reagents. The entire procedure can be completed in 1 hour without phenol/chloroform extraction. Purified DNA is suitable for use in PCR or other enzymatic reactions.
DNA Isolation Dual Kit (Plant) Sample: 100 mg of fresh plant tissue or 25 mg of dry plant tissue Format: Reagent and spin column Operation time: within 90 minutes For research use only Introduction The DNA Isolation Dual Kit (Plant) combines reagent system and spin column system in it. The kit was designed specifically for genomic DNA isolation from plant samples. This unique reagent system ensures total DNA with high yield and good quality from samples and the spin column system was designed to purify or concentrate DNA products which have been previously isolated using Reagents. The entire procedure can be completed in 1 hour without phenol extraction. Purified DNA is suitable for use in PCR or other enzymatic reactions.
l pH/mV/Temp儀器 l 150組記憶 l 最大最小測量值記憶 l 斜率%顯示 l 5點內建認知校正點 l 附玻璃電極 l 溫度測棒 l 電極夾座 l 附校正標準液各乙瓶 l (USB輸出、工作平台攪拌器選購)
l pH/mV/Temp/DO/O2儀器 l USB輸出 l 150組記憶 l 最大最小測量值記憶 l 斜率%顯示 l 5點內建認知校正點 l 附玻璃電極 l 溫度測棒 l 電極夾座 l 附校正標準液各乙瓶 l 溶氧手調鹽度及高度 (工作平台攪拌器選購)。
l pH/mV/Temp/Cond/TDS/Salt/DO/O2儀器 l USB輸出 l 150組記憶 l 最大最小測量值記憶 l 斜率%顯示 l 5點內建認知校正點 l 附玻璃電極 l 溫度測棒 l 電極夾座 l 附校正標準液各乙瓶 l 溶氧手調鹽度及高度 l (工作平台攪拌器選購)
pH/mV/Temp/Cond/TDS/Salt儀器 l USB輸出 l 150組記憶 l 最大最小測量值記憶 l 斜率%顯示 l 5點內建認知校正點 l 附玻璃電極 l 溫度測棒 l 電極夾座 l 附校正標準液各乙瓶 l (工作平台攪拌器選購)。
商品名稱: HAMILTON 微量注射器(可換式針頭)-Microliter Syringe◆特色>GC用注射器>針頭可更換 商品名稱: HAMILTON 微量注射器(固定式針頭)-Microliter Syringe◆特色>GC用注射器>針頭固定於注射器上不可更換>有平針頭(SNR系列)與尖針頭(N系列)2種 ◆型錄下載
SC475 Polypropylene Digestion Vessels >Certified for low metals background >Lined closure system ensures leak-free performance with no contamination >Highly accurate graduation eliminate need for graduated cylinders >Compact packaging reduces shipping costs SC475 digestion vessels provide premium performance in your SC154 HotBlock™, SC100 HotBlock or AutoBlock™. These vessels are molded of clarified homopolymer polypropylene assuring higher working temperatures and greater chemical resistance than the commonly used co-polymer polypropylene. Vessels are graduated to 50mL and have a total 68mL capacity. The molded-in graduations are extremely accurate. Analysts can easily reproduce volumes to within 0.5% mrrting the quantitative demands of the laboratory. Using the vessels' graduations for sample measurement not only reduces the need for graduated cylinders and volumetric flasks, but also eliminated sample transfers. This results in a "one-cup" system that greatly reduces labor and costs associated with metals perperation. The 33mm threaded cap is lined with a 0.040" polyethylene faced foam liner for a leak-proof seal. Only the metals-free polyethylene liner contacts the sample, not the cap itself.SC475 digestion vessels provide premium performance in your SC154 HotBlock™, SC100 HotBlock or AutoBlock™. These vessels are molded of clarified homopolymer polypropylene assuring higher working temperatures and greater chemical resistance than the commonly used co-polymer polypropylene. Vessels are graduated to 50mL and have a total 68mL capacity.The 33mm threaded cap is lined with a 0.040 polyethylene-faced foam liner for a leak-proof seal. Only the metals-free polyethylene liner contacts the sample. 500 vessels with caps. Total volume of the vessel is 68ml.Each case comes with a Certificate of Analysis For Cleanliness and Volume Accuracy.